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How To Fruit All In One Grow Bags

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Cultivating mushrooms at home has become a popular activity for gardeners and food enthusiasts alike. One of the most convenient methods of mushroom cultivation is using an all-in-one grow bag. These bags are pre-sterilized and come with grain that’s ready to inoculate with your mushroom spores or culture and substrate that’s ready for your mycelium to grow into to provide moisture for fruiting. Here’s how to fruit mushrooms using an all-in-one grow bag, with some tips to ensure a successful harvest!

Understanding All-in-One Grow Bags

Before you start, it’s crucial to understand what an all-in-one grow bag is. These bags typically contain a blend of grains and a substrate like hardwood sawdust or CVG, which provide the necessary nutrients and moisture for mushrooms to thrive. They are designed to simplify the mushroom cultivation process, making it accessible even to beginners, and making things fast and easy for experienced growers.

Benefits of Using Grow Bags

  • Sterility: Pre-sterilized and self-contained to minimize contamination risks.
  • Convenience: Contains everything needed for the mushroom life cycle, hands off allowing you to have very little interaction with your project.
  • Efficiency: Simplifies the process, saving time and effort.

Preparing Your Grow Bag

First, ensure that you have a high-quality all-in-one mushroom grow bag. Look for suppliers who have positive reviews and offer detailed instructions with their products. Avoid vendors who use flashy graphics to advertise bad products hoping that they’ll catch your eye to get your money. Also avoid bags that are premixed which indicates the vendor is skipping steps and making inferior products compared to layered all-in-one bags. 


  • Start by inoculating your grow bag with mushroom spores or a liquid culture syringe.
  • Inject the spores or culture into the grain through the injection port.


  • After inoculation, store the bag at the appropriate temperature for the species you’re growing—usually between 70-75°F.
  • Keep the bag out of direct sunlight and check for signs of colonization after 14 days.

Fruiting Your Mushrooms

Once the bag is fully colonized, it’s time to introduce fruiting conditions.

Introducing Fruiting Conditions

  • Lower the temperature to the fruiting range for your mushroom species, generally between 68-70°F.
  • Provide indirect light for 12  hours each day to encourage mushrooms to grow.

Harvesting Your Mushrooms

When mushrooms have fully developed and before the caps fully uncurl, it’s time to harvest.

  • Gently twist and pull the mushrooms from the substrate.
  • Harvest in batches as not all mushrooms will develop at the same pace.

Maintaining Your Grow Bag

After the first flush of mushrooms, your all-in-one grow bag can often produce multiple harvests.

  • Continue to mist the substrate and provide fresh air. Mist the tops of the inside of the bag once a day, then roll the top of the bag closed. 
  • Wait for subsequent flushes, which may be smaller than the first but still rewarding. Subsequent flushes typically take 1-2 weeks to begin appearing after harvesting.

Tips for Success

  • Sanitation: Always work with clean hands and tools to prevent contamination.
  • Patience: Mushroom growth can be slow; give your bag time to colonize and fruit.
  • Monitoring: Watch for any signs of contamination, such as odd smells or colors, and address them promptly.

By following these steps, you should be well on your way to enjoying fresh, homegrown mushrooms right from your all-in-one grow bag. With a little care and attention, you can reap the delicious rewards of mushroom cultivation.

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