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All-In-one grow Bag instructions

Step 1

Choose and clean your work area. If you’re using a still air box, clean the inside thoroughly, wiping the inside surface and lid with 70% isopropyl alcohol or bleach wipes. If you are not working in a still air box, choose a room with little to no air movement. Clean the surface you’re working on, then clean the outside of the bag including the black injection port. 

Step 2

Once your alcohol or bleach has dried from the bag, attach the needle to your syringe. Make sure that it is completely attached before removing the syringe cap. 

Step 3

Remove the syringe cap and use a lighter to heat the metal syringe tip to red hot. Once red, stop heating, you do not want to overheat this part of the syringe, since the attachment piece is plastic, you do not want to melt the plastic portion. 

Step 4

Allow your syringe tip to cool from red hot. DO NOT WIPE OR TOUCH THE NEEDLE WITH ANYTHING. After the syringe is no longer red, wait about 3 seconds, then insert into the rubber injection port. 


Once inside the port, inject 2-4cc of genetic material into your bag moving your needle around as you inject. If you inject more than 2-4cc that is fine, but it is unnecessary. If using only 1 bag for your syringe, you can inject up to 10cc of genetic material, though this is unnecessary. 



Step 5

Withdraw your syringe, recap the needle, and if reusing your genetic material syringe replace the syringe cap. If not reusing, dispose of your syringe and needle appropriately. 

Step 6

Allow your grain to colonize. This can take anywhere from 2-4 weeks depending on the genetics you’ve chosen, and the conditions you’re growing in. You want to keep the bag in an area that is dark and 70-75°. If your grow area is cooler than this, you’ll want to use a heat mat. For heat mats put your grow bag inside of a tote or box, then place that on a towel, then place the tote or box on top of the heat mat. The other option is to put the heat mat inside the box or tote next to your grow bag, avoiding having the bag on top of the mat or having the mat touch your bag. Doing this prevents the bottom of your bag warming up while the top remains cool, and the bottom of the bag drying out. Do not put your bag directly on a heating mat, this will dry your bag out fairly quickly, rendering it useless. 

You should only see white mycelium growing in your grain. If you see cloudy moisture around your grains, different colors (red, purple, yellow, green, blue, etc), or black growth, you should dispose of your bag and start again as these are contaminants and not what you’re looking to grow. If you have any questions about what’s growing in your bag, please contact us with pictures of your questionable growth.

Patience is key here. If you inject your genetic material and don’t see any growth after 2 weeks, wait 2 more weeks! Don’t fiddle with your bag every other day. Mycelium grows in extremely small fiberous strands, by picking up and moving the bag you risk damaging the strands of growth, repeatedly damaging the growth will cause it to stall and stop growing. After injecting leave the bag alone for a minimum of 2 weeks before looking at it. 

We do not warranty other vendor’s genetics. If genetics that you added to the bag do not grow or grow poorly, you would want to contact the vendor you purchased the genetic material from. 

Step 7

Once your grain is between 50-80% colonized (white) break up the colonized grain thoroughly, and begin mixing into the substrate layer. It may help to lay the bag flat on its side and roll the material to mix. Once thoroughly mixed, hold your bag by the top portion and shake to get the material stuck in the top loose, pat the bottom of the bag to help dislodge anything remaining. Some material will remain which is alright as long as there’s no grains lingering above. Once all your material is back at the bottom of the bag, place on a surface and slightly press on the top and sides to slightly compact your substrate block. 

Step 8

Allow your substrate to colonize. This can again take anywhere from 2-4 weeks. You want to keep your bag in the same conditions as before, 70-75°F in darkness. Once fully colonized, you can do a few different things.
You can cut the top seal of your bag, allowing fresh air to get into your bag. This will signal to your bag where fresh air is coming from, allow CO2 to leave the bag, and give your mycelium fresh air which will initiate fruiting. If you do this, you will need to mist the top portion of the bag at least once a day, and you may want to keep the bag inside of a plastic tote to ensure the mycelium in the bag does not dry out or get too much fresh air. 
If growing oyster or lions mane mushrooms, the best option is to cut 2 1-2 inch crossing lines forming an X into the short sides of your bag (avoid cutting in too deep, you’re just cutting the plastic, not the substrate itself), then fold the top of your bag over, pushing the air inside out. This will allow your mushrooms to fruit from the holes you cut instead of from the top of the block, resulting in better looking fruits. Doing this method you will want to spray the holes you cut 2-3 times a day with a misting of water to ensure nothing dries out. This method you can do in an area with medium to low air movement, or inside of a tote box. 

Step 8.1

The next  method you can try is removing the bag entirely and fruiting your bag as a cake. Remove the bag, and place your block inside of a tub lined with moist perlite to maintain humidity in the tub. Give your block fresh air at least 2 times a day now by fanning the top of the tote pushing fresh air into it. You don’t want a lot of air movement because this will dry your fruits out, but you want them to be able to breath.


If top fruiting (the top seal of the bag has been cut) you want to have humidity on the sides of the bag above your substrate. If you do not see this humidity, mist the inside of your bag once a day with a spray bottle, but don’t spray the surface directly. If side fruiting, once you see your fruits beginning to form, mist them once or twice daily depending on how much airflow you have. You do not want your fruits to become dry, but you do not want them to be overly wet. For the cake method, make sure humidity is on the sides of the tote, if there’s not mist the sides, but don’t directly spray the block.

Step 8.2

The last method and easiest method for fruiting is to do nothing. With this method you can even skip the substrate mixing step. You can simply inject, wait, and eventually you’ll harvest. Some varieties won’t fruit well with this method, others will. This method can also take a bit longer, 6-8 weeks is not unheard of, but it is the absolute easiest method! 


Typically mushrooms can be harvested by grabbing the base of the fruit, twisting and pulling in one motion. This can cause a bit of the substrate to come detached with your fruits, so some people prefer to use scissors to cut the base of the mushroom. Either method is fine. Once you have harvested, you can dispose of your block in your garbage or (preferably) in a compost pile/container. Another option is to dunk your substrate. If you top fruited your bag, you can fill your bag with cold water until the top of the substrate is covered, let sit for 1 hour, then drain and repeat step 8. If you side fruited your bag, you can place your bag in a bowl or pan, fill that with water, then let sit for 1 hour, remove the block, then drain through the side hole you previously cut, then repeat step 8. Second flushes are typically much smaller (50% or less) than the first flush.

I can only find 90%/99% isopropyl alcohol, is that okay?

Yes that is fine, you will want to wipe your surfaces twice, or water down your alcohol by ~1/3rd of the volume of the container.

I melted the rubber injection port by injecting with too hot of a needle! What do I do?!

Just put a piece of tape over the port, this is not a major issue. Tape it sooner than later though, as soon as possible after you see you’ve melted the port.

My mycelium is growing slowly, what gives?

Sometimes mycelium grows slow! If using a spore syringe, it can take 1-2 weeks to see growth at all because the spores have to germinate.

There’s something green/blue/purple/grey/yellow growing in my bag!

That’s likely a contaminant. Mold, bacteria, and other funguses are all ubiquitous in the air around us. It’s possible your genetics had something in them they shouldn’t have, you didn’t wipe the injection port well enough, or you did not flame sterilize your needle properly. Best advice here is to dispose of your bag in the trash, or cut it open and dump it in your compost. Contaminants can be dangerous, it’s best to dispose of things when you first identify them rather than let them continue to grow.

What temperature should I keep my bag in? Does humidity matter? Lighting?

We recommend keeping our bags in an environment between 70-75°F. This is the optimal temperature range for mycelium to flourish. Humidity is not an important factor because the bag is contained. You do not need to worry about maintaining humidity unless you cut open the bag or remove the block from the bag. Lighting is also not an important factor, you can have a light on a timer for 12 hours on 12 hours off, or 18 on 6 off. Mushrooms are not photosynthetic but they are photosensitive, meaning they don’t need light to grow but they will react to light usually in the form of growing towards the light. Do not keep your bag in direct sunlight as strong light can damage mycelium (it’s typically underground or contained within trees).

Nothing is growing?

Sometimes genetics take longer to start than others. When working with liquid culture, you should see growth within 2-4 days of injection. When working with spores, you might not see growth for 2 weeks! Patience is key here. If you wait more than 2 weeks and don’t see any growth, you can either wait another 2 weeks, or you can use new genetics in the bag. It’s very unlikely that after 2 weeks the first genetics will start growing, and as long as the bag hasn’t contaminated, it’s the same as injecting the bag with water and maybe some sugars, so adding new genetics won’t be detrimental.
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